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Systematic evaluation of the DNA repair machinery facilitated the development...

Chen(Q),Lyu(L),Wang(S),Zhao(ZK) Synth Syst Biotechnol 2027-03-00

...CRISPR-Cas9 system was used to introduce double-strand breaks (DSBs), HR efficiency was dramatically increased to 16%, a...

Strategy for enhanced production of A40926B0 in Nonomuraea gerenzanensis usin...

Wang(X),Zhai(H),Gu(D),Wang(Y),Gao(J),Du(L)... Synth Syst Biotechnol 2027-04-00

...CRISPR/AsCas12f1 genome editing system and applied systematic metabolic engineering to boost A40926B0 biosynthesis. Firs...

CRISPR-Cas9 mediated adiA knockout in Hafnia paralvei: Implications for agmat...

Casado(A),Wellner(SM),Quirós(A),Herrero-Fr... Food Microbiol 2027-01-00

...CRISPR-Cas9 system, marking the first successful application of this technology in the genus Hafnia. Moreover, the adiA ...

Autosomal dominant gain-of-function mutations in LCP1 cause a syndromic neutr...

Yu(L),Zhou(B),Liu(W),Li(W),Wei(Q),Zhang(Y)... Genes Dis 2027-01-00

...CRISPR-edited cells, and LCP1+/L362F mice replicate these defects. Mechanistically, mutant LCP1 hyper-bundles F-actin, i...

The precision strategy of human genome correction via a set of circular donor...

Kusano(K),Takizawa(K),Kawaguchi(J),Hara(I)... Front Genome Ed None

...CRISPR-Cas9, can trigger a copy-paste-type HR event; however, CRISPR-Cas9 more frequently induces imprecise non-homologo...

In vivo multiomic Perturb-seq with enhanced nuclear gRNA capture.

Zheng(X),Li(J),Kim(K),Simmons(SK),Zhao(Z),... bioRxiv 2026-03-17

...CRISPR screening with joint transcriptomic and chromatin readouts has been limited by inefficient recovery of gRNAs from...

Using image classifiers to predict CMT2A disease-relevant mitochondrial motil...

Epstein(L),Weiner(AC),Macklin(BL),Kelly(KR... bioRxiv 2026-03-17

...CRISPR-based gene editing technologies holds promise for screening new therapies at scale in vitro. A critical roadblock...

Parkinson's disease linked LRRK2 G2019S drives oxidative nuclear DNA damage a...

Liu(J),Gonzalez-Hunt(CP),Richbourg(T),Barr... bioRxiv 2026-03-01

...CRISPR/Cas9-generated LRRK2G2019S/G2019S knock-in cells, we discovered increased sensitivity to oxidative and alkylating...

ULK1 drives NDP52-mediated selective autophagic degradation of MHC-I to promo...

Vu(L),Giacobbi(NS),Khalil(MI),Yang(C),Ecke... bioRxiv 2026-03-17

...CRISPR screens, we identified components of the ULK1 and PIK3C3 complexes for autophagy initiation complexes among the t...

A mouse model of autosomal dominant spastic ataxia and myopathy caused by a m...

Hines(TJ),Funke(JR),Pratt(SL),Rice(AD),Twi... bioRxiv 2026-03-09

...CRISPR-engineered into C57BL/6J mice, which confirmed the Tuba4a Q176P variant as the causative mutation. Mutant mice ar...

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