Whole exome sequencing in SMRad51 and Control primary mouse embryonic fibroblasts

Homologous recombination (HR) is a Rad51-mediated evolutionary conserved process that plays a unique role in genome plasticity, controlling the balance genetic stability/diversity. In this study we compared the induction of genetic modifications by whole exome sequencing in primary mouse embryonic fibroblasts after the expression of a dominant negative form of Rad (SMRad51). We used a transgenic mouse embryonic fibroblasts in which SMRad51 expression was induced under doxycycline (Dox) control. We compared SMRad51 primary mouse embryonic fibroblasts treated with Dox (transgene expression induced) with control mouse embryonic fibroblasts treated with Dox.