mRNA expression profiles of SK-N-AS neuroblastoma cells with induced ALK-wt, ALK-F1174L or ALK-R1275Q

·发布 2015年5月4日 ·更新 2015年5月4日

样本数

实验数

芯片平台

相关文献

实验描述

Establishment of inducible SK-N-AS ALKwt, ALKF1174L and ALKR1275Q cell lines Human neuroblastoma SK-N-AS cells were electroporated with pcDNA6/TR (Invitrogen) using the Neonå¨ Transfection System (Life Techologies). Single cell clones were generated using blasticidin (7.5åµg/ml) and limited dilution. Using a TetR antibody (Clonetech), the clone with the highest TetR expression was selected (named SK-N-AS-TR) and used for the transfection with pT-REx-DEST30-ALK, ALKF1174L or ALKR1275Q. After transfection of SK-N-AS-TR with the ALK variants, single cell clones were raised using geneticin (500 åµg/ml) and limited dilution, while blasticidin treatment was continued as described above. Total RNA of (treated) cell lines, transgenic mice tumors and ganglia was isolated using the miRNeasy kit (Qiagen) according to the manufacturer‰۪s instructions, including on-column DNase treatment. Samples were labeled and hybridised to the Sureprint G3 human GE or G3 Mouse GE 8x60K microarrays (Agilent Technologies), according to the manufacturer‰۪s guidelines and starting from 200 ng of RNA.

参考文献

Upregulation of MAPK negative feedback regulators and RET in mutant ALK neuroblastoma: implications for targeted treatment

Irina Lambertz1*, Candy Kumps1*, Shana Claeys1, Sven Lindner2, Anneleen Beckers1, Els Janssens1, Daniel R. Carter3, Alex Cazes4, Belamy B. Cheung3, Marilena De Mariano5, An De Bondt6, Sara De Brouwer1, Olivier Delattre4, Jay Gibbons7, Isabelle Janoueix4, Genevi̬ve Laureys8, Chris Liang7, Glenn M. Marchall3, Michael Porcu9, Junko Takita10, David Camacho Trujillo1, Ilse Van Den Wyngaert6, Nadine Van Roy1, Alan Van Goethem1, Tom Van Maerken1, Piotr Zabrocki9, Jan Cools9, Johannes H. Schulte2,11,12,13,14, Jorge Vialard15, Frank Speleman1**$, Katleen De Preter1**

PMID: 25805801

芯片平台

A-MEXP-2102

Agilent 028004 SurePrint G3 Human GE 8x60K Microarray（36 例）

样本属性

cell line

SKNAS-TR-ALK-F1174L, SKNAS-TR-ALK-R1275Q, SKNAS-TR-ALK-wt

filename

US45103088_252800419754_S01_GE1_105_Dec08_1_1.txt, US45103088_252800419754_S01_GE1_105_Dec08_1_2.txt, US45103088_252800419754_S01_GE1_105_Dec08_1_3.txt, US45103088_252800419754_S01_GE1_105_Dec08_1_4.txt, US45103088_252800419754_S01_GE1_105_Dec08_2_1.txt, US45103088_252800419754_S01_GE1_105_Dec08_2_2.txt, US45103088_252800419754_S01_GE1_105_Dec08_2_4.txt, US45103088_252800419755_S01_GE1_105_Dec08_1_1.txt, US45103088_252800419755_S01_GE1_105_Dec08_1_2.txt, US45103088_252800419755_S01_GE1_105_Dec08_1_3.txt, US45103088_252800419755_S01_GE1_105_Dec08_2_2.txt, US45103088_252800419755_S01_GE1_105_Dec08_2_3.txt, US45103088_252800419755_S01_GE1_105_Dec08_2_4.txt, US45103088_252800419758_S01_GE1_105_Dec08_1_1.txt, US45103088_252800419758_S01_GE1_105_Dec08_1_2.txt, US45103088_252800419758_S01_GE1_105_Dec08_1_3.txt, US45103088_252800419758_S01_GE1_105_Dec08_2_2.txt, US45103088_252800419758_S01_GE1_105_Dec08_2_3.txt, US45103088_252800419758_S01_GE1_105_Dec08_2_4.txt, US45103088_252800419759_S01_GE1_105_Dec08_1_1.txt, US45103088_252800419759_S01_GE1_105_Dec08_1_2.txt, US45103088_252800419759_S01_GE1_105_Dec08_1_3.txt, US45103088_252800419759_S01_GE1_105_Dec08_2_1.txt, US45103088_252800419759_S01_GE1_105_Dec08_2_2.txt, US45103088_252800419759_S01_GE1_105_Dec08_2_3.txt, US45103088_252800419759_S01_GE1_105_Dec08_2_4.txt, US45103088_252800419760_S01_GE1_105_Dec08_1_1.txt, US45103088_252800419760_S01_GE1_105_Dec08_1_2.txt, US45103088_252800419760_S01_GE1_105_Dec08_1_3.txt, US45103088_252800419760_S01_GE1_105_Dec08_1_4.txt, US45103088_252800419763_S01_GE1_105_Dec08_1_3.txt, US45103088_252800419763_S01_GE1_105_Dec08_1_4.txt, US45103088_252800419763_S01_GE1_105_Dec08_2_1.txt, US45103088_252800419763_S01_GE1_105_Dec08_2_2.txt, US45103088_252800419763_S01_GE1_105_Dec08_2_3.txt, US45103088_252800419763_S01_GE1_105_Dec08_2_4.txt

organism

homo sapiens

time

12 hour, 24 hour, 48 hour

实验信息

登记号

E-MTAB-3207

实验类型

transcription profiling by array

物种

homo sapiens

发布日期

2015年5月4日

更新日期

2015年5月4日

提交者

Katleen De Preter

外部链接

ArrayExpress 原始页

分析服务

mRNA expression profiles of SK-N-AS neuroblastoma cells with induced ALK-wt, ALK-F1174L or ALK-R1275Q

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