mRNA expression profiles of TAE-684 treated or shALK transfected neuroblastoma cell lines

·发布 2015年5月4日 ·更新 2015年5月4日

113

样本数

113

实验数

芯片平台

相关文献

实验描述

Human wild-type ALK (SK-N-AS, NGP, IMR-32), ALKR1275Q (CLB-GA, LAN-5, UKF-NB-3), ALKF1174L (SK-N-SH, Kelly, SMS-KCNR) and ALK amplified (NB-1) neuroblastoma cell lines were treated in triplicate with 0.3ë_M NPV-TAE-684 (Novartis/SelleckChem) or DMSO (VWR) for 6 hours, followed by RNA isolation and gene expression profiling. For shRNA mediated knockdown, pGIPZ-ALK shRNAmir and pGIPZ-non-silencing control shRNAmir vectors were used (Open Biosystems). The ALK shRNA was directed against a part of exon 26 in the tyrosine kinase domain of ALK (target sequence: TGGAAGGAATATTCACTTCTAA). Lentiviral particles were produced according to manufacturer‰۪s protocol (Open Biosystems). On day 2 post-transduction of neuroblastoma cells, the transduction efficiency was determined by flow cytometry and microscopic analysis of GFP-positive cells (>90% so no further selection was performed). Cells were subsequently harvested for expression profiling. Labeling of the RNA samples and hybridisation to the Affymetrix HG-U133plus2 arrays was performed using the manufacturer‰۪s protocol starting from 1 åµg of RNA.

参考文献

Upregulation of MAPK negative feedback regulators and RET in mutant ALK neuroblastoma: implications for targeted treatment

Irina Lambertz1*, Candy Kumps1*, Shana Claeys1, Sven Lindner2, Anneleen Beckers1, Els Janssens1, Daniel R. Carter3, Alex Cazes4, Belamy B. Cheung3, Marilena De Mariano5, An De Bondt6, Sara De Brouwer1, Olivier Delattre4, Jay Gibbons7, Isabelle Janoueix4, Genevi̬ve Laureys8, Chris Liang7, Glenn M. Marchall3, Michael Porcu9, Junko Takita10, David Camacho Trujillo1, Ilse Van Den Wyngaert6, Nadine Van Roy1, Alan Van Goethem1, Tom Van Maerken1, Piotr Zabrocki9, Jan Cools9, Johannes H. Schulte2,11,12,13,14, Jorge Vialard15, Frank Speleman1**$, Katleen De Preter1**

PMID: 25805801

芯片平台

A-AFFY-44

Affymetrix GeneChip Human Genome U133 Plus 2.0 [HG-U133_Plus_2]（113 例）

样本属性

cell line

CLBGA, IMR32, KELLY, LAN5, NB1, NGP, SKNAS, SKNSH, SMSKCNR, UKFNB3

disease

neuroblastoma

genotype

ALKamplification, ALKF1174L, ALKR1275Q, ALKwt

10457.CEL, 10458.CEL, 10459.CEL, 10460.CEL, 10461.CEL, 10462.CEL, 10463.CEL, 10464.CEL, 10465.CEL, 10466.CEL, 10467.CEL, 10468.CEL, 10469.CEL, 10470.CEL, 10471.CEL, 10473.CEL, 10474.CEL, 10475.CEL, 10476.CEL, 10477.CEL, 10478.CEL, 10479.CEL, 10480.CEL, 10481.CEL, 10482.CEL, 10483.CEL, 10484.CEL, 10485.CEL, 10486.CEL, 12862.CEL, 12863.CEL, 12864.CEL, 12865.CEL, 12866.CEL, 12867.CEL, 12868.CEL, 12869.CEL, 12870.CEL, 12871.CEL, 12872.CEL, 12873.CEL, 12874.CEL, 12875.CEL, 12876.CEL, 12877.CEL, 12878.CEL, 12879.CEL, 13416.CEL, 13417.CEL, 13418.CEL, 13419.CEL, 13420.CEL, 13421.CEL, 13422.CEL, 13423.CEL, 13424.CEL, 13425.CEL, 13426.CEL, 13427.CEL, 13428.CEL, 13429.CEL, 13430.CEL, 13431.CEL, 13432.CEL, 13433.CEL, 13434.CEL, 13435.CEL, 13436.CEL, 13437.CEL, 13438.CEL, 13439.CEL, 13446.CEL, 13447.CEL, 13448.CEL, 13449.CEL, 13450.CEL, 13451.CEL, 13452.CEL, 13453.CEL, 13454.CEL, 13455.CEL, 13456.CEL, 13457.CEL, 17152.CEL, 17153.CEL, 17154.CEL, 17155.CEL, 17156.CEL, 17157.CEL, 17158.CEL, 17159.CEL, 17160.CEL, 17161.CEL, 17162.CEL, 17163.CEL, 8849.CEL, 8850.CEL, 8851.CEL, 8852.CEL, 8853.CEL, 8854.CEL, 8855.CEL, 8856.CEL, 8857.CEL, 8858.CEL, 8859.CEL, 8860.CEL, 8861.CEL, 8862.CEL, 8863.CEL, 8864.CEL, 8865.CEL, 8866.CEL

organism

Homo Sapiens

实验信息

登记号

E-MTAB-3205

实验类型

transcription profiling by array

物种

Homo Sapiens

发布日期

2015年5月4日

更新日期

2015年5月4日

提交者

Katleen De Preter

外部链接

ArrayExpress 原始页

分析服务

mRNA expression profiles of TAE-684 treated or shALK transfected neuroblastoma cell lines

联系地址

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