Single-cell total RNA sequencing (VASA-seq) on index-sorted human colonic chip infected by wild-type S.Tm

We infected adult human intestine epithelium stem cell derived colonic chip with wild-type S.Tm 14028 with GFP label. To link intracellular replication levels to host response, we performed single-cell total RNA sequencing (VASA-seq) on index-sorted cells from GFPLow, GFPInt and GFPHigh levels.