RNA sequencing–based determination of the RNA interactome of wild-type AKAP1 and its deletion mutants deficient for the KH or Tudor domain.

AKAP1 is highly expressed in glioblastoma and is required for tumor growth. We therefore analyzed the AKAP1 RNA interactome in glioblastoma cells. Native RNA immunoprecipitation followed by high-throughput sequencing (RIP-Seq) was performed in U87MG cells transiently expressing V5-tagged mouse wild-type AKAP1, as well as deletion mutants lacking either the KH (Δ563–630) or Tudor (Δ709–786) domain, to assess domain-specific RNA binding.