Systematic annotation of orphan RNAs reveals blood-accessible molecular barcodes of cancer identity and cancer-emergent oncogenic drivers

The purpose of this experiment is to identify cellular processes that are associated with cellular expression of oncRNAs, which are cancer-emergent non-coding, small RNAs. To test this, we compared the transcriptomes of our breast cancer cells lines (MDA-MB-231 and HCC-LM2) overexpressing oncRNA.ch7.29 or oncRNA.ch17.67 relative to respective controls. Cell lines were transduced with shctrl, oncRNA.ch7.29, or oncRNA.ch17.67 under the control of a U6 promoter. RNA was extracted from the cell lines and used as input for mRNA-seq library construction.