Epigenomic characterization of patient-derived lung tumoroids using DNA methylation profiling

DNA methylation analysis of patient-derived lung tumoroids and matched healthy organoids demonstrates preservation of tumor-specific epigenomic identity during long-term culture. Tumoroids maintain the methylation landscape of their parental tumors, with samples from the same patient clustering together based on DNA methylation profiles regardless of culture conditions. Differential methylation analysis reveals distinct tumor-specific patterns distinguishing tumoroids from healthy organoids, confirming stable epigenomic preservation. These findings validate the tumoroid platform as a reliable model for precision CAR T-cell therapy development and personalized treatment testing in lung cancer.