TCR sequencing of pathogenic and non pathogenic Th1 and Th17 cells subsets

Th1 and Th17 cell subsets were sort-purified (FACSAria III, BD Biosciences, or Aurora CS, Cytek) from peripheral blood of HD and CF patients, or from lung-tissues of CF and non-CF patients, according to the expression of specific surface markers combination among CD4+IL-7R+CD25low cells: CCR6-CXCR3+ (Th1 cells), CCR6+CXCR3-CCR5- (cTh17 cells), CCR6+CXCR3-CCR5+ (pTh17 cells), CCR6+CXCR3+CCR5- (Th1/17- cells), CCR6+CXCR3+CCR5+ (Th1/17+ cells).