miRNA-seq of exosomes derived from bone-metastatic lung cancer cells

This study aimed to identify miRNAs enriched in exosomes that promote osteolytic bone metastasis. We performed miRNA-seq directly on exosomes purified from the culture supernatants of either non-metastatic (NC-LLC) or bone-metastatic (BM-LLC) Lewis lung carcinoma cells. The goal was to profile the miRNA cargo of these tumor-derived exosomes and identify key regulators of intercellular communication. Integrated analysis with mRNA sequencing data from recipient macrophages identified exosomal miR-484 as a critical driver of osteoclast differentiation and bone metastasis.