Genome-wide CRISPR knockout screen to identify genes involved in macrophage differentiation

·发布 2026年4月29日

样本数

实验数

相关文献

实验描述

To identify genes involved in macrophage differentiation, a genome-wide CRISPR knockout library in macrophage progenitors was differentiated into macrophages. Samples were collected from the progenitor library at day 0, from a library maintained as progenitors for seven days, and from macrophages after seven days of differentiation.

参考文献

Genome-wide screen in Mycobacterium tuberculosis infected macrophages reveals innate regulation of antibacterial mediators by IRF2

Allison W. Roberts, Linda N. Del Cid, Nicholas E. Garelis, Jeffery S. Cox

样本属性

Genetic modification

genome-wide CRISPR knockout library: Brie (Doench et al 2016) in lentiGuide-puro

Cell type

macrophages

Cell line

cas9-expressing, HoxB8 conditionally-immortalized macrophages

Organism

Mus musculus

Developmental stage

mouse postnatal

Disease

normal

Genotype

cas9-expressing C57BL/6

Organism part

bone marrow

Stimulus

undifferentiated, passaged in media with beta-estradiol, undifferentiated, macrophage differentiation

Replicate

2, 3, 1

实验信息

登记号

E-MTAB-15740

实验类型

DNA-seq

物种

Mus musculus, Mus musculus

发布日期

2026年4月29日

提交者

Allison Roberts、 Jeffery Cox

外部链接

ArrayExpress 原始页

分析服务

Genome-wide CRISPR knockout screen to identify genes involved in macrophage differentiation

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