Single cell RNAseq of haploidized HCT116 colorectal carcinoma cells expressing specific p53 mutations treated with either DMSO or Nutlin

·发布 2024年10月16日

样本数

实验数

实验描述

Colorectal carcinoma cell line HCT116 cells were used as a model system to investigate the heterogeneity of different TP53 mutations. To this end, cells were haploidized by deleting an intronic splice site in one of the two wild-type TP53 alleles while the other copy was altered to different loss-of-function mutants via CRISPR/Cas9 gene editing. Cells expressing different haploid TP53 genotypes were treated with either Nutlin or DMSO to investigate the genetic variation between the mutants and the wild-type genotype.

样本属性

Organism

Homo sapiens

Developmental stage

adult

Disease

colorectal carcinoma

Genotype

mixed

Organism part

colorectum

Cell type

colorectal cancer cell

Cell line

HCT116

实验信息

登记号

E-MTAB-13904

实验类型

RNA-seq of coding RNA from single cells

物种

Homo sapiens, Homo sapiens

发布日期

2024年10月16日

提交者

Marco Mernberger、 Julianne Funk、 Maria Klimovich、 Daniel Drangenstein、 Ole Pielhoop、 Pascal Hunold、 Anna Borowek、 Maxim Noeparast、 Evangelos Pavlakis、 Michelle Neumann、 Dimitrios-Ilias Balourdas、 Katharina Kochhan、 Nastasja Merle、 Imke Bullwinkel、 Michael Wanzel、 Sabrina Elmshäuser、 Julia Teply-Szymanski、 Andrea Nist、 Tara Procida、 Marek Bartkuhn、 Katharina Humpert、 Rajkumar Savai、 Thierry Soussi、 Andreas Joerger、 Thorsten Stiewe

外部链接

ArrayExpress 原始页

分析服务

Single cell RNAseq of haploidized HCT116 colorectal carcinoma cells expressing specific p53 mutations treated with either DMSO or Nutlin

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