RNAseq of murine small intestinal organoid cultures treated with Type 1 (IFNg) or Type 2 (IL4+IL13) cytokines in the presence or absence of excretory/secretory (ES) products of the helminth parasite Heligmosomoides polygyrus

Heligmosomoides polygyrus is a natural intestinal parasite of mice which exerts wide ranging modulatory effects on the immune system. This experiment was designed to investigate its abillity to modify intestinal epithelial cells, which form part of its natural niche. We tested gene expression in vitro, in differentiating organoids of small intestinal origin, exposed to cytokines and the released products of the parasite, termed HpES.