Reconstructing the RUNX2 transcriptional hierarchy in thyroid cancer using a multi-layered network- based approach (ChIP-seq Histone Markers)

We performed Chromatin Immunoprecipitation followed by deep-sequencing in TPC1 thyroid cancer cell line model, in order to profile the genomic distribution of H3K27ac, H3K4me1 and H3K4me3, epigenetic markers of chromatin functional status. These data were integrated with RUNX2 genomic occupancy to define the nature and the activation status of the RUNX2-associated regions.