E-GEOD-39166
SRP014145, GSE39166
ChIP-seq
Schizosaccharomyces pombe
Strand-specific ChIP-seq profiling of Rad52 localization
·Released July 7, 2012
·Updated March 11, 2013
66
Samples
66
Assays
Description
We report the development of a novel strand-specific ChIP-seq strategy and application of this strategy in studying genome instability events We examined vulnerable genomic sites by applying strand-specific ChIP-seq of Rad52 to S. pombe haploid mutants showing elevated level of genome instability.
Sample Attributes
chip antibody
None, Anti-FLAG M2 Affinity Gel, GFP-Trap, IgG Sepharose 6 Fast Flow
genotype
None, h+ leu1-32 his3-D1 arg3::HOsite-natMX nmt1terminator::[pJR-41XH+HO] rad22-2xFLAG-kanMX, h+ leu1-32 ura4-D18 his3-D1 arg3-D4 (hht1-hhf1)::his3 (hht1-hhf3)::arg3 hhf2::ura4 hst4::kanMX rad22-CFP-TAP-natMX, h+ leu1-32 ura4-D18 hst4D::kanMX rad22-YFP-kanMX, h+ leu1-32 ura4-D18 pfh1-R20 rad22-CFP-TAP-natMX ura4 marker replacing a potential origin between SPBTRNAGLN.01 and SPBTRNAGLN.03, h+ leu1-32 ura4-D18 pfh1-R20 SPATRNAVAL.01::ura4 rad22-CFP-TAP-natMX, h+ leu1-32 ura4-D18 rtt109D::kanMX rad22-CFP-TAP-natMX, h+ ura4-D18 leu1-32 his3-D1 rad22-CFP-TAP-natMX, h- leu1-32 ams2D::kanMX rad22-CFP-TAP-natMX, h- leu1-32 cbp1D::kanMX rad22-CFP-TAP-natMX, h- leu1-32 his3-D1swi3D::kanMX rad22-CFP-TAP-natMX, h- leu1-32 hst4D::kanMX cdc25-22 rad22-CFP-TAP-natMX, h- leu1-32 hst4D::kanMX mms22D::hphMX rad22-CFP-TAP-natMX, h- leu1-32 hst4D::kanMX rad22-CFP-TAP-natMX, h- leu1-32 pfh1-R20 rad22-CFP-TAP-natMX, h- leu1-32 pfh1-R23 rad22-CFP-TAP-natMX, h- leu1-32 rad22-CFP-TAP-natMX, h- leu1-32 swi1D::kanMX rad22-CFP-TAP-natMX, h- leu1-32 ura4-D18 his3-D1 arg3-D4 (hht1-hhf1)::his3 (hht1-hhf3)::arg3 hhf2::ura4 rad22-CFP-TAP-natMX Otr1R::ade6, h- leu1-32 ura4-D18 his3-D1 arg3-D4 (hht1-hhf1)D::his3 (hht3-hhf3)D::arg3 Tf2-12-neoAI::ura4 rad22-CFP-TAP-natMX, h- leu1-32 ura4-D18 hst4D::kanMX rad11-TAP::kanR, h- smt0 leu1-32 ura4-D18 rad22-CFP-TAP-natMX, h- ura4-D18 leu1-32 his3-D1 arg3-D4 (hht1-hhf1)D::arg3 hhf2::ura4 Otr1:ade6 rad22-CFP-TAP-natMX, h- ura4-D18 leu1-32 his3-D1 arg3-D4 (hht3-hhf3)D::arg3 hhf2::ura4 Otr1:ade6 rad22-CFP-TAP-natMX, h- ura4-D18 leu1-32 his3-D1 arg3-D4 ade6-M210 (hht1-hhf1)D::his3 (hht3-hhf3)D::arg3 hhf2K56Q hst4::kanMX rad22-CFP-TAP-natMX, h- ura4-D18 leu1-32 his3-D1 arg3-D4 ade6-M210 (hht1-hhf1)D::his3 (hht3-hhf3)D::arg3 hhf2K56R hst4D::kanMX rad22-CFP-TAP-natMX, h- ura4-D18 leu1-32 his3-D1 arg3-D4 ade6-M210(hht1-hhf1)D::his3 (hht3-hhf3)D::arg3 hhf2K56A hst4D::kanMX rad22-CFP-TAP-natMX, h-leu1-32 rtt109D::kanMX hst4D::kanMX rad22-CFP-TAP-natMX
Organism
Schizosaccharomyces pombe
Experiment Info
Accession
E-GEOD-39166
GEO ID
SRP014145, GSE39166
Type
ChIP-seq
Organism
Schizosaccharomyces pombe
Released
July 7, 2012
Updated
March 11, 2013
Submitter
Zhi-Xiong Zhou、
Zhi-Xiong Zhou、
Li-Lin Du、
Mei-Jun Zhang、
Xu Peng
Analysis Services
Contact
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